FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing.

FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing.

Blog Article

Massively parallel sequencing gotrax handlebar of cell-free, maternal plasma DNA was recently demonstrated to be a safe and effective screening method for fetal chromosomal aneuploidies.Here, we report an improved sequencing method achieving significantly increased throughput and decreased cost by replacing laborious sequencing library preparation steps with PCR employing a single primer invertatop squeeze bottle pair designed to amplify a discrete subset of repeated regions.Using this approach, samples containing as little as 4% trisomy 21 DNA could be readily distinguished from euploid samples.